■ 基本信息
別名: | PB200PA-1 |
啟動(dòng)子: | Polr2A |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類: | 哺乳細(xì)胞轉(zhuǎn)座酶表達(dá)質(zhì)粒 |
質(zhì)粒大小: | 8531bp |
原核抗性: | Amp |
克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 哺乳細(xì)胞 |
誘導(dǎo)方式: | 無(wú)須誘導(dǎo)，瞬時(shí)表達(dá) |
5'測(cè)序引物: | M13R:CAGGAAACAGCTATGACC |
3'測(cè)序引物: | 根據(jù)序列設(shè)計(jì)引物 |
備注: | 轉(zhuǎn)座酶哺乳細(xì)胞表達(dá)質(zhì)粒 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 蛋白表達(dá),轉(zhuǎn)座 |
片段類型: | ORF
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片段物種: |
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原核抗性: | Amp |
真核抗性: |
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
Super PiggyBac Transposase (PB200PA-1)是一個(gè)哺乳細(xì)胞轉(zhuǎn)座質(zhì)粒。The PiggyBac (PB) transposon is a mobile genetic element that efficiently transposes between vectors andchromosomes via a "cut and paste" mechanism. During transposition, the PB transposase recognizes transposonspecific inverted terminal repeat sequences (ITRs) located on both ends of the transposon vector and efficiently moves the contents from the original sites and efficiently integrates them into TTAA chromosomal sites. The powerful activity of the piggyBac transposon system enables genes of interest between the two ITRs in the PB vector to be easily mobilized into target genomes.The Super PiggyBac transposase expression vector features a 5’ HS4 insulator to support robust transcription from the rPolr2A promoter. The PiggyBac transposase coding sequence has been optimized for high expression, stability and activity in mammalian cells.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK565Super PiggyBac Transposase (PB200PA-1) 哺乳轉(zhuǎn)座質(zhì)粒.txt
