■ 基本信息
啟動子: | PC,polyhedrin |
復制子: | pUC ori，F(xiàn)1 ori |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類: | 廣宿主系列，昆蟲細胞載體 |
質(zhì)粒大小: | 4858bp |
質(zhì)粒標簽: | N-His；N-TEV |
原核抗性: | Amp |
真核抗性: | Gen |
克隆菌株: | DH5α |
培養(yǎng)條件: | 37℃，有氧 LB |
表達宿主: | 昆蟲細胞 |
誘導方式: | 無須誘導，瞬時表達 |
5'測序引物: | pFastbac-F: TATTCCGGATTATTCATACC |
3'測序引物: | pFastBac-R: ACAAATGTGGTATGGCTGA |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 昆蟲細胞 |
質(zhì)粒用途: | 蛋白表達 |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
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熒光標記: |
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■ 質(zhì)粒簡介
The pFastBac HT vector is supplied with the multiple cloning site in three reading frames (A, B, and C) to facilitate cloning your gene of interest in frame with the N-terminal 6xHis tag.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK2327pFastBacHTC昆蟲胞內(nèi)質(zhì)粒.txt
